One QTL named qCT6.1 was repeatedly detected. Genetic analysis of the chilling injury index (CII) of the RILs population showed that cold tolerance in cucumber seedlings was quantitatively inherited. In this study, a 186 recombinant inbred lines (RILs) population constructed by crossing two inbred lines, cold-tolerant ‘CG104’ and cold-sensitive ‘CG37’, was used to identify loci associated with cold tolerance in cucumber seedlings. Cucumber seedlings exposed to extreme cold stress seriously reduce cucumber yield, however, few cold-tolerant genes have been identified yet. These results provide a basis for the fine mapping of salt tolerant genes and further study of the molecular mechanism of salt tolerance in cucumber seedlings.Ĭucumber (Cucumis sativus L.), an essential and economically important vegetable crop, is sensitive to cold stress. Subsequent qRT-PCR showed that the relative expression levels of both genes during salt treatment were significantly different between the two parents. Among these genes, Csa6G487740 and Csa6G489940 showed variations in amino acid sequence between lines CG104 and CG37. It was delimited into a 1397.1 kb region, and nine genes related to salt tolerance were identified. In experiments carried out in April and July 2019, qST6.2 on chromosome six was repeatedly detected. The results showed that salt tolerance in cucumber seedlings is a quantitative trait controlled by multiple genes. At the same time, the candidate genes within the mapping region were cloned and analyzed. In this study, the recombinant inbred line (RIL) population (from a cross between the salt tolerant line CG104 and salt sensitive line CG37) was used to study the genetic mechanism of salt tolerance in cucumber seedlings. Cucumber is very sensitive to salt stress, and excessive salt content in soils seriously affects normal growth and development, posing a serious threat to commercial production.
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